Specimen Requirements

Title

VIRUS CULTURE

Specimen Requirement

Specimen source required.
Swab specimens in viral transport medium.
Biopsy/tissue specimens in saline or viral transport medium. (snap frozen -20°C).
Nasal washes in sterile, leak proof sterile container, 2.0 mL (1.0 mL minimum).
Nasal aspirates in vacuum trap, 1.0 mL (0.5 mL minimum).
Body fluids undiluted in sterile leak-proof container. 2.0 mL (1.0 mL minimum).
Stool in sterile leak-proof container, 2.0 mL (1.0 mL minimum). Liquid stool or marble size solid stool. Stool in Cary blair medium.
Undiluted urine in sterile leak-proof container, 2.0 mL (1.0 mL minimum), for Mumps only.
Bronchoalveolar lavage/wash in sterile leak-proof container, 2.0 mL (1.0 mL minimum).
Note: CVD is the preferred method.
Blood in EDTA (lavender top) tube, 3.0 mL (2.0 mL minimum). Note: CVD is the preferred method.

 

Methodology

Stability

Reference Range

Turnaround

Up to 14 days

Clinical Use

This procedure wil not reliably detect cytomegalovirus (CMV), enteroviruses, noroviruses, respiratory syncytial virus (RSV), and varicella zoster (VZV), Comprehensive Virus Detection (CVD) or the individual PCR test is recommended. See CYPCR, EVCR, NOPCR, RSPCR and VVPCR. CVD is strongly recommended for blood, any body fluid, bronchoalveolar lavage/wash, and aspirates. Urine specimens are acceptable for Mumps detection only. CSF is not acceptable for Virus Culture.

Test Code

VCULT