Specimen Requirements


Testosterone, Free, Bioavailable and Total, MS

Specimen Requirement

Collect red top, promptly centrifuge, transfer 2.8 mL serum to a screw top plastic vial, indicate sample type on tube and send.

Patient preparation:
Administration of STRENSIQ may interfere in certain assays and may falsely elevate values. For patients receiving STRENSIQ, consideration should be given to using alternate methods.


Chromatography/Mass Spectrometry • Calculation (CALC) • Spectrophotometry (SP) • Immunochemiluminescent Assay


Room temp: 7 days, Refrigerated: 7 days, Frozen: 28 days

Reference Range

By Report*


4-6 days

Clinical Use

Includes: Testosterone Total, Testosterone Free, Testosterone Bioavailable, Sex Hormone Binding Globulin, Albumin.

Helpful in assessing testicular function in males and managing hirsutism, virilization in females.

Test Code