PM/Scl-100 Ab (RDL)
Collect SST/1.0 mL serum, allow to clot for 30 minutes, promptly centrifuge and send.
Alternate sample: Red top. 1.0 mL Serum must be separated and transferred to a screw top transport tube within 1 hour of collection, indicate sample type on tube before sending.
Enzyme-linked immunosorbent assay (ELISA)
Room temp: 7 days, Refrigerated: 14 days, Frozen: 60 days
The anti-PM/Scl-100 antibody is associated with younger age, calcinosis and has lower rates of gastrointestinal symptoms, ILD and pulmonary hypertension. There is also evidence of a possibly better survival compared to the presence of either anti-PM/Scl-75 or anti-Scl-70 antibodies.