Specimen Requirements

Title

HCV Genotype 1 NS5a Drug Resistance

Specimen Requirement

Preferred specimen: Collect PPT or SST, centrifuge and send.

Alternate collection: Collect lavender EDTA, centrifuge, transfer 2.0 mL plasma to a screw top plastic vial, indicate sample type on tube and send.

Order this test for a patient with a genotype 1a, if genotype is unknown, order test code HCVGQ for HCV Genotype first.

Methodology

Polymerase chain reaction (PCR) amplification and DNA next generation sequencing (NGS)

Stability

Room Temperature: 72 hours; Refrigerated: 14 days; Frozen: 42 days

Reference Range

The reference range is determined by the performing laboratory and will show on the report.

Turnaround

5-10 days (if HCVGQ is also ordered, turn around will be delayed)

Clinical Use

Assessment of drug susceptibility by nucleic acid sequencing of a patient’s hepatitis C virus (HCV) to the NS5A inhibitors. Detects resistance associated variants at positions 28, 30, 31, and 93 in genotype 1a HCV.

 

MUST HAVE GENOTYPE 1a!!!

Test Code

HCGT1