Specimen Requirements
Title
HCV Genotype 1 NS5a Drug Resistance
Specimen Requirement
Preferred specimen: Collect PPT or SST, centrifuge and send.
Alternate collection: Collect lavender EDTA, centrifuge, transfer 2.0 mL plasma to a screw top plastic vial, indicate sample type on tube and send.
Order this test for a patient with a genotype 1a, if genotype is unknown, order test code HCVGQ for HCV Genotype first.
Methodology
Polymerase chain reaction (PCR) amplification and DNA next generation sequencing (NGS)
Stability
Room Temperature: 72 hours; Refrigerated: 14 days; Frozen: 42 days
Reference Range
The reference range is determined by the performing laboratory and will show on the report.
Turnaround
5-10 days (if HCVGQ is also ordered, turn around will be delayed)
Clinical Use
Assessment of drug susceptibility by nucleic acid sequencing of a patient’s hepatitis C virus (HCV) to the NS5A inhibitors. Detects resistance associated variants at positions 28, 30, 31, and 93 in genotype 1a HCV.
MUST HAVE GENOTYPE 1a!!!
Test Code
HCGT1