Specimen Requirements

Title

INTRINSIC FACTOR BLOCKING ANTIBODY

Specimen Requirement

DRAW BLOOD IN A SST/1.0 mL.  SPIN, SEPARATE SERUM FROM CELLS WITHIN 2 HOURS.

VERY HIGH SERUM LEVELS OF VITAMIN B12 MAY GIVE FALSE POSITIVE RESULTS FOR INTRINSIC FACTOR ANTIBODY. NO SAMPLE SHOULD BE COLLECTED FROM A PATIENT WHO HAS RECEIVED VITAMIN B12 INJECTION WITHIN THE PAST WEEK.

Methodology

Stability

ROOM TEMP 8 HOURS; REFRIGERATED 48 HOURS

Reference Range

BY REPORT

Turnaround

3-5 DAYS pERFORMED SUN, TUES, THURS

Clinical Use

Differentiation of pernicious anemia from other megaloblastic anemias. Intrinsic factor blocking autoantibodies are found in approximately 75% of patients with pernicious anemia. High serum concentrations of vitamin B12 (cobalamin) cause false positive results.

Test Code

IFA