Specimen Requirements

Title

HIV-1 Genotype

Specimen Requirement

Collect PPT/2.0 mL plasma, centrifuge and send.

Alternate sample: Lavender EDTA, centrifuge, pipette 2.0 mL plasma to a screw top transport tube, indicate sample type on tube and send.

Methodology

DNA Sequencing • Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)

Stability

Room temp: 24 hours, Refrigerated: 6 days, Frozen: 42 days

Reference Range

By Report*

Turnaround

4-9 days

Clinical Use

This test is intended for use in conjunction with clinical presentation and other laboratory markers of disease progress for the clinical management of HIV-1 infected patients. The test identifies drug resistance-associated mutations in the HIV-1 protease and reverse transcriptase genes. It can be used to predict antiretroviral drug resistance before initiation of therapy and in patients experiencing virologic failure while on therapy.

Test Code

GTHIV