Anti-ENA Antibodies (ANTI-RNP/ANTI-Sm)
Collect SST or red top/1 mL serum. If SST collected, allow to clot for 30 minutes, centrifuge and send refrigerated.
Enzyme Linked Immunoassay
Room temp: 8 hours, Refrigerated: 7 days, Frozen: 14 days
Detect antibodies associated with SLE and mixed connective tissue disease. Anti-Sm is highly specific for SLE. Anti-RNP is found with a variety of rheumatoid diseases with high titers associated mainly with MCTD