Specimen Requirements



Specimen Requirement

Collect SST or red top. After 30 minutes of clotting, promptly centrifuge and send 0.8 mL serum. If collecting red top, transfer serum to a screw top plastic transport tube, indicate sample type on tube and send.

Patient should fast for 14 to 16 hours for basal values.

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R).



Room temp: 1 day, Refrigerated: 14 days, Frozen: 14 days

Reference Range

By Report*


1 day

Clinical Use

The principal use of C-peptide is in the evaluation of hypoglycemia. Patients with insulin-secreting neoplasms have high levels of both C-peptide and endogenous insulin; in contrast, patients with factitious hypoglycemia will have low C-peptide levels in the presence of elevated (exogenous) serum insulin. C-peptide is also useful in evaluating residual beta-cell function in insulin-dependent diabetics, many of whom have antibodies that interfere with insulin assays. Glucagon-stimulated C-peptide concentration has been shown to be a good discriminator between insulin-requiring and non−insulin-requiring diabetic patients. The diagnosis of islet cell tumor is supported by elevation of C-peptide when plasma glucose is low.

Test Code