Specimen Requirements


Bile Acids, Fractionated & Total

Specimen Requirement

Collect SST/1 mL serum. After 30 minutes of clotting, promptly centrifuge and send.

Alternate sample: Red top.

Patient Preparation: Overnight fasting is preferred.


Liquid Chromatography/Tandem Mass Spectrometry


Room Temperature: Unacceptable; Refrigerated: 7 days; Frozen: 30 days

Reference Range

By Report*


5-7 days, Performed Sunday-Friday

Clinical Use

Bile acids are formed in the liver, from cholesterol, stored and concentrated in the gallbladder, and excreted into the intestines in response to food. Elevated concentrations often suggest impaired hepatic clearance due to liver disease. Patients with intestinal malabsorption and metabolic disorders such as Gilbert’s disease do not exhibit elevated bile acids concentrations.

Test Code