Specimen Requirements

Title

ANTI-NEUTROPHIL CYTOPLASMIC ANTIBODY (ANCA)

Specimen Requirement

1 SST/2.0 mL SERUM.  AFTER 30 MINUTES OF CLOTTING, PROMPTLY CENTRIFUGE AND REFRIGERATE SERUM. (INCLUDES EVALUATION FOR P-ANCA, C-ANCA AND ATYPICAL P-ANCA)

Methodology

Stability

ROOM TEMPERATURE: 7 DAYS; REFRIGERATED: 14 DAYS; FROZEN: 30 DAYS

Reference Range

BY REPORT*

Turnaround

WITHIN 3 DAYS/SET UP: TUES-SAT A.M.

Clinical Use

C-ANCA are present in 84-100% of patients with generalized Wegener granulomatosis (WG). C-ANCA-positive sera containing PR-3 Abs are 80% sensitive and 97% specific for WG. Positive ANCA results obtained in patients with other types of vasculitis typically give a PANCA pattern and are usually associated with autoantibodies to myeloperoxidase (MPO).

Test Code

ANCAQ