Specimen Requirements


Amikacin, Serum

Specimen Requirement

1.0 mL serum (0.2 mL minimum) from a Non-Barrier Red Top. (Codes below) Collect trough just before next dose; 30-45 minutes after IV dose or 1-2 hours after IM dose for peak. Please indicate on requisition if patient is taking penicillin or cephalosporins. After 30 minutes of clotting, promptly centrifuge, separate and refrigerate serum within 2 hours.



Room Temp Unacceptable; Refrigerated 14 days; Frozen 14 days

Reference Range

By report*


1-2 days

Clinical Use

Amikacin is used to treat serious infections caused by susceptible aerobic gram-negative bacilli (eg, a number of the Enterobacteriaceae, P. aeruginosa). Such infections include bacteremias; intra-abdominal, soft tissue (including burns), bone or joint, lower respiratory tract, and complicated urinary tract infections, and meningitis. The major advantage of amikacin is its superior resistance profile. Thus, it is the aminoglycoside of choice for infections caused by aerobic gramnegative bacilli that are known or suspected to be resistant to other aminoglycosides. It should be the aminoglycoside of choice for general use in hospitals where the prevalence of resistance to gentamicin and tobramycin is known to be high or when there is concern about gentamicin- and tobramycin-resistant gram-negative bacilli in a particular clinical situation.

Test Code